Journal: Biosensors & bioelectronics
Article Title: Customizable OpenGUS immunoassay: A homogeneous detection system using β-glucuronidase switch and label-free antibody.
doi: 10.1016/j.bios.2024.116796
Figure Lengend Snippet: Fig. 6. Detection of analytes in complex matrices using customized OpenGUS immunoassays. (A) Correlation of the Cry j 1 concentrations determined by the OpenGUS immunoassay and ELISA. Reaction time of the OpenGUS immunoassay was 15 min. (B) Correlation of the recovered and designated hCRP concentrations. Reaction time of the OpenGUS immunoassay was 15 min. (C) Distinguish two groups of human saliva samples containing normal and high level of hLF. The fluorescence increase rates were determined by fitting the F.I. of 5, 10, 15, 20 min to a linear equation. Each biological sample (Normal-level group: hLF ≤14.9 μg/ mL, n = 14; High-level group: hLF >14.9 μg/mL, n = 9) was measured in technical triplicates (n = 3). *: Welch’s t-test, p < 0.05.
Article Snippet: A human lactoferrin ELISA kit (catalog No. EL2011-1) was purchased from Assay Pro (USA).
Techniques: Enzyme-linked Immunosorbent Assay, Fluorescence